I would like to know if anyone of you has recombinantly produced the protease Factor Xa using E. coli expression system in your lab. I know some labs produce and purify their own proteases like TEV and Thrombin. I am trying to purify an MBP tagged protein using a pMAL-c5X expression vector. So, my protein construct has an MBP tag which can be cleaved with only Factor Xa protease enzyme.
I would be very grateful if you could share your knowledge and expertise to help me out. Thank you for your time and help.
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Protease factor Xa production and purification in E. coli
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